Kategórie

Klonovací systém pSpark® TA Done

DNA cloning

Na objednávku

Výrobca: CANVAX

Katalógové číslo: C0021-S

Na sklade 0 Položka

Obr.	Názov variantu	Cena	Stav	Objednať
	Počet reakcií : 5 rxn	0,00 €	Skladom
	Počet reakcií : 10 rxn	99,33 €	Na objednávku
	Počet reakcií : 20 rxn	197,51 €	Na objednávku

Popis

pSpark® TA Done DNA Cloning Kit

For Efficient, Stable & Easy cloning of blended and non-proofreading PCR fragments with EcoRI and NotI flanking the insertion site

Product Overview:

pSpark® TA Done is efficient, stable and easy-to-use DNA cloning vector based on an improved TA technology that offers all of the advantages of pSpark® TA with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion

Advantages & Features

Convenient: recognition sites for EcoRI and NotI flanking the insertion site.
Flexible: allows removing the desired insert DNA with other restriction digestion.
Efficient: more than 600 white positive colonies expected under optimal conditions.
Stable: without cloning bias due to transcription of toxic genes.
Easy-to-use: eliminates screening of recombinants due to its low background (4%).
Fast protocol: ligation time from 60 minutes to overnight.
Compatible: with direct cloning of PCR products.
Great versatility: compatible with any competent cell, primer design or particular proofreading DNA Polymerase.
Cost avoidance: avoids primer phosphorylation.
Risk-free: product covered by our Quality 100% Guarantee.