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pMBL™ T-Vector DNA Cloning Kit
For a Efficient, Convenient & Fast Cloning of DNA fragments with A overhangs
Product Overview:
pMBL™ T-Vector DNA Cloning Kit is an efficient, convenient and fast system for the cloning of PCR products. The vector is prepared by cutting pMBL™ T-Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmids by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases such as Horse-Power™ Taq DNA Polymerase.
These Polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments.
Advantages & Features
- Highly efficient: greater than 90% white colonies in a transformation with supplied insert control.
- Proven performance: more than 1,000 recombinant colonies expected in optimal conditions.
- Fast and easy protocol: results from 15 min protocol.
- Optimized: improve efficiency of ligation of a PCR product into the plasmid.
- Compatible: overhang for ligation of PCR products preventing recircularization of the vector.
- Designed by cutting the vector with EcoRV and adding a 3´ terminal thymidine to both ends.
- Risk-free: product covered by our Quality 100% Guarantee.