Exonuclease I (E. coli), 20,000U

Exonuclease I (E. coli), 20,000U

From E. coli (20 U/μL)

Na sklade 1 ks
Výrobca: CANVAX
Katalógové číslo: EZ0017
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258,50 €
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235,00 € bez DPH

Exonuclease I (E. coli)

From E. coli (20 U/μL)

Product Overview:

Exonuclease I, the product of the sbcB gene of E. coli, is an exodeoxyribonuclease that hydrolyzes single-stranded DNA (ssDNA) stepwise in a 3′→5′ direction releasing 5′-mononucleotides and leaving the terminal 5′-dinucleotide intact.

Advantages & Features

  • High Specific Activity: 185,000 U/mg.
  • 3′ -> 5′ single strand exonuclease.
  • Versatile: suitable with a wide-range of Buffer conditions.
  • Compatible with Magnesium.
  • Complete solution: supplied with 10x Exonuclease I Reaction Buffer.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Unit Definition:

One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37 °C in 1X Exonuclease I Reaction Buffer with 0.17 mg/ml single-stranded [3H]-DNA.

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