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DNase I
RNAse-Free (10 mg/mL)
Product Overview:
DNase I is a recombinant endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products (on average producing tetranucleotides) with 5’-phosphorylated and 3’-hydroxylated ends.
It acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids and chromatin. DNase I requires bivalent cations (Mg2⁺ and Ca2⁺) for maximal activity.
Advantages & Features
- Recombinant: bovine Pancreatic, purified from E. coli (29 kDa monomer).
- High specific activity: more than 2,000 Kunitz units per mg protein.
- High Quality: free of RNase and Proteinase contamination.
- Complete solution: supplied with 10x Reaction Buffer.
- Risk-free: product covered by our Quality 100% Guarantee.
Specifications
Unit definition: One Kunitz unit is defined as the amount of enzyme required for the complete degradation of 1 µg of plasmid DNA in 10 minutes at 37 ˚C.
Note: One Kunitz unit equals to 50 Worthington units.