aloquence Kategórie

ONPG – Galactosidase Assay Kit , 500 assays

ONPG – Galactosidase Assay Kit , 500 assays

Optimized, Stable & Convenient tool to quickly measure the levels of active β-galactosidase

Na objednávku
Výrobca: CANVAX
Katalógové číslo: CA080

Na vyžiadanie

Kontaktujte nás

Upozorniť ma pri dostupnosti

ONPG – Galactosidase Assay Kit

Optimized, Stable & Convenient tool to quickly measure the levels of active β-galactosidase

Product Overview:

ONPG B-Galactosidase Assay Kit is an optimized, stable and convenient tool to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.

Lac Z is often used reporter gene in experiments transfection because the β-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. β-galactosidase performs the hydrolysis of orthonitrophenyl-β-D-galactopyranoside (ONPG) to the ortho-nitrophenol (ONP). This ONP produces a bright yellow colour that is detected at an absorbance of 420 nm. The concentration of β-galactosidase is proportional to colour produced.

Advantages & Features

  • Proven performance to quantify high expression level of beta-Gal.
  • Very stable: resistant to proteolytic degradation and easily assayed.
  • Convenient for all transfection assays.
  • Versatile: proven performance for cultured cells and tissues.
  • Fast and easy protocol.
  • Cost-effective.
  • Risk-free: product covered by our Quality 100% Guarantee.
Produkt bol pridaný do zoznamu prianí