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PAS-AB technique is used to differentiate between acidic mucins as sialomucins, sulfomucin and neutral epithelial mucin. After staining, acidic mucins will be coloured blue, neutral in magenta and nuclei in deep blue. Mixtures of acidic and neutral mucins give blue/ purple colour.
Uses and s ample preparation:
Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining.
Components
Schiff Reagent
Periodic acid 0,5%
Harris Hematoxylin
Alcian Blue
Short procedure
Place in Alcian Blue
Wash in running tap water and distilled water
Place in periodic acid 0.5%
Rinse with running tap water and distilled water
Apply Schiff?s Reagent
Rinse with running tap water and distilled water
Apply Hematoxylin
Rinse with tap water
Dehydrate with graded ethanol, clear and cover
Staining characteristics:
Acidic mucins can be coloured in blue, neutral in magenta and nuclei in deep blue. Mixtures of acidic and neutral mucins give blue/ purple colour.