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Hematoxylin Eosin is a common staining technique used in a wide range of tissues. Through this method, nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple.
Uses and sample preparation:
Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.
Components
Mayer?s Hematoxylin Reagent
Eosin Reagent
Short procedure
Add Hematoxylin
Wash with warm tap water
Add Glacial acetic acid
Wash with tap water
Add Eosin solution
Wash with tap water
Dehydrate with graded ethanol, clear and coverslip
Staining characteristics:
Nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple