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PRIEMYSEL
- ! VÝPREDAJ !
Highly Purified (>98%) for Routine Amplifications in Ready-to-use format
Horse-Power™ Taq DNA Polymerase MasterMix (2x)
Highly Purified (>98%) for Routine Amplifications in Ready-to-use format
Product Overview:
Horse-Power™ Taq DNA Polymerase MasterMix (2x) is an optimized ready-to-use MasterMix that contains all PCR reaction components: dNTPs, PCR buffer, Mg2+ and Taq DNA polymerase. Only primers and template need to be added. The convenient MasterMix (2x) formulation saves time and eliminates the risk of contamination due to a reduced number of pipetting steps required.
Horse-Power™ Taq DNA Polymerase is a versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).
Advantages & Features
- Highest purity: greater than 98% confirmed by SDS-PAGE.
- Highest quality: high activity, specificity, thermostability and performance in PCR.
- Highly efficient: reactivation buffer improved.
- Thermostable: half-life at 94 ºC is 40 minutes.
- Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
- Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc.
- Molecular Weight: 94 kDa.
- Convenient: available in different concentrations, sizes and solutions.
- Risk-free: product covered by our Quality 100% Guarantee.
Specifications
Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.
Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.