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M-MLV Reverse Transcriptase (200 u/uL), 50,000 U

Encoded Moloney Murine Leukemia Virus, purified from E.coli (200 U/µL)

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Výrobca: CANVAX

Katalógové číslo: P0074

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M-MLV Reverse Transcriptase

Encoded Moloney Murine Leukemia Virus, purified from E.coli (200 U/µL)

Product Overview:

M-MLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNAdependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized to single-stranded DNA.

Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of MMLV-RT.

MW: 69 KDa.

Advantages & Features

Concentration: 200 U/µL.
Full Solution: includes Reaction Buffer 5x.
High Quality: free of DNAse, RNase or protease contamination.
Purity: >90% as judged by SDS-polyacrylamide gels with blue staining.
Final Product Size: 7 kb or less.
Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Unit definition:

One unit of the enzyme incorporates 1 nmol dTTP into acid-precipitable material in 10 minutes at 37 ° C, using poly(A) oligo dT as a template prime