Kategórie

AMV Reverse Transcriptase (20 u/uL), 1,000 U

From Avian Myeloblastosis Virus, purified from E.coli

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Výrobca: CANVAX

Katalógové číslo: P0071-CS

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AMV Reverse Transcriptase

From Avian Myeloblastosis Virus, purified from E.coli

Product Overview:

AMV Reverse Transcriptase, encoded by Avian Myeloblastosis Virus (AMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template.

AMV Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids. The enzyme possesses an intrinsic RNase H activity. AMV RT possesses multiple enzymatic activities including RNA- and DNA-directed DNA polymerase, DNA-RNA unwinding activity, a sequence- specific Mn2+ -dependent endonuclease and ribonuclease H.

Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of AMLV-RT.

Advantages & Features

Concentration: 200 u/µL
Full Solution: includes Reaction Buffer 5x.
High quality: free of RNase, and DNase (exo- and endonuclease) activities.
Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Unit definition:

One unit is the amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide into acid-
precipitable material in 10 minutes at 37 °C, using poly(A) oligo dT as a template primer.