pOnebyOne III - Retroviral, 10 rxn

pOnebyOne III - Retroviral, 10 rxn

Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest

Na objednávku
Výrobca: CANVAX
Katalógové číslo: ME0015-S

pOnebyOne™ III – Retroviral Bicistronic Mammalian Expression Kit

Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest

Product Overview:

pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technologyThe expression cassette of pOnebyOne™ III – Retroviral incorporates the Human elongation factor 1 alpha promoter that precedes 2A sequence in frame with the truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones. It can be visualized by cytometry using a specific antibody labelled with FITC or using a similar method and also, it can be enriched from negative clones with magnetic beads bearing an antiΔNGFR antibody

Advantages & Features

  • Complete solution: a directional cloning vector to clone and produce a protein of interest.
  • Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter).
  • Highly efficient: cloning system tested with up to 4 kb inserts.
  • Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment.
  • Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest.
  • Cost avoidance: avoids the use of restriction enzymes.
  • Accurate: proven performance for most common cell lines.
  • Flexible: allows transfection in difficult cell lines.
  • Really low experimental background: lower than 1%.
  • Convenient: available with different resistance marker cassettes.
  • Directional cloning: of PCR with the gene of interest.
  • Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

  • Prom (Citomegalovirus promoter): 8-567
  • 5´LTR: 574-748
  • Ψ+/ ΔGAG packaging signal: 749-1747
  • Human elongation factor 1 alpha  promoter: 1755-2956
  • 2A from equine rhinitis A virus: 2966-3028
  • ΔNGFR: 3035-3870
  • 3´LTR SIN (self-inactivated deletion): 3926-4352
  • Ampicillin resistance gene (ORF): 5306-6162
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