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PRIEMYSEL
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Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest
pOnebyOne™ III – Retroviral Bicistronic Mammalian Expression Kit
Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest
Product Overview:
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ III – Retroviral incorporates the Human elongation factor 1 alpha promoter that precedes 2A sequence in frame with the truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones. It can be visualized by cytometry using a specific antibody labelled with FITC or using a similar method and also, it can be enriched from negative clones with magnetic beads bearing an antiΔNGFR antibody
Advantages & Features
- Complete solution: a directional cloning vector to clone and produce a protein of interest.
- Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter).
- Highly efficient: cloning system tested with up to 4 kb inserts.
- Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment.
- Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest.
- Cost avoidance: avoids the use of restriction enzymes.
- Accurate: proven performance for most common cell lines.
- Flexible: allows transfection in difficult cell lines.
- Really low experimental background: lower than 1%.
- Convenient: available with different resistance marker cassettes.
- Directional cloning: of PCR with the gene of interest.
- Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.
- Risk-free: product covered by our Quality 100% Guarantee.
Specifications
- Prom (Citomegalovirus promoter): 8-567
- 5´LTR: 574-748
- Ψ+/ ΔGAG packaging signal: 749-1747
- Human elongation factor 1 alpha promoter: 1755-2956
- 2A from equine rhinitis A virus: 2966-3028
- ΔNGFR: 3035-3870
- 3´LTR SIN (self-inactivated deletion): 3926-4352
- Ampicillin resistance gene (ORF): 5306-6162