Bst DNA Polymerase 8 U/uL

Bst DNA Polymerase 8 U/uL

Ready for LAMP (Loop-mediated Isothermal Amplification)

Na objednávku
Výrobca: CANVAX
Katalógové číslo: P0045

Obr. Názov variantu Cena Stav Objednať
Balenie : 2000 U
136,29 €
Na objednávku
Balenie : 10000 U
478,17 €
Na objednávku

Bst DNA Polymerase (Exonuclease Minus)

Ready for LAMP (Loop-mediated Isothermal Amplification)

Product Overview:

Large Fragment of Bst DNA Polymerase, Exonuclease Minus, is isolated from a Bacillus stearothermophilus. It catalyzes 5’ 3’ synthesis of DNA and lacks 5’ 3’ and 3’ 5’ exonuclease activities.

Source:  A recombinant E. coli strain carrying the Bst DNA Polymerase gene (Large fragment).

Advantages & Features

  • High Purity: greater than 99% confirmed by SDS PAGE.
  • Reverse transcription activity.
  • Optimized: Increased activity.
  • Strong strand displacement activity.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Unit definition:
One unit catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65 C in 20 mMTris-HCl pH 8.8, 10 mM (NH4)2SO4,10 mM KCl, 2 mM MgSO4, 0.1% Triton X-100, 30 nM M13mp18 ssDNA, 70 nMM13 sequencing primer(-47) 24-mer 200 uM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP) and 0.1 mg/mL BSA.

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