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PRIEMYSEL
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For a Reliable, Robust & highly Efficient Site-directed Mutagenesis based in PCR
PickMutant™ Site-directed Mutagenesis Kit
For a Reliable, Robust & highly Efficient Site-directed Mutagenesis based in PCR
Product Overview:
PickMutant™ is a reliable, robust and highly efficient PCR-based Mutagenesis Kit. Extremely easy-to-use, the kit allows creating single or multiple point mutations, deletions or insertions using a rapid and easy protocol.
All these mutation could be obtained by PCR using a FastPANGEA™ High Fidelity DNA Polymerase and well-designed Mutagenesis primers. The assembled mutagenic PCR fragments is cloned into Canvax™ pSpark® Cloning vector, specially designed to clone blunt PCR fragments with high efficiency or into other vector designing, in this case, an additional specific vector primer pair.
Advantages & Features
- Highly Effective point mutations (single or multiple), deletions or insertions.
- Easy and fast protocol: it takes less than 3 hours in one step procedure.
- Cost avoidance: compatible with any bacterial strains or primers.
- Versatile: compatible with any Cloning vector.
Includes
Includes for 15 rxn:
– 150 μL Proofreading DNA Polymerase MasterMix (2x)
– 300 U Glue Enzyme (10 U/μL)
– 40 μL Glue Enzyme Buffer (10x)
– 5 μL Insert Control DNA
– 15 μL pSpark® I (20 ng/μL)
- Efficient: includes highly efficient pSpark® to clone blunted fragments.
- Robust: simultaneous assemble and clone of PCR fragments.
- Risk-free: product covered by our Quality 100% Guarantee.