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For a highly Efficient & Versatile Protein Expression (LIC technology based) in E. coli from PCR fragment cloned
pColiExpress™ IV LIC Cloning & Expression Kit
For a highly Efficient & Versatile Protein Expression (LIC technology based) in E. coli from PCR fragment cloned
Product Overview:
pColiExpress™ IV LIC Cloning & Expression Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family systems are based in directional ligase-independent-cloning (LIC) technology, a rapid procedure that provides high-cloning efficiencies and the fast production of a large quantity of any desired protein.
It contains all elements required for the expression of large quantities of any desired protein by the T7 RNA Polymerase Inducible System, and a His6 tag at COOH end of the protein that allows the purification. It contains at the NH2 end the Cmyc tag followed by a 3C from human Rhinovirus (HRV) Protease cleavage site, too. The vector has also an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.
Advantages & Features
- Ready-to-use vectors.
- Highly efficient cloning system.
- Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
- Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
- Minimum background: lower than 1%.
- Time-saving protocol: avoids any step required after PCR.
- Higher protein expression than other suppliers.
- Cost avoidance: avoids the use of expensive phosphorylated primers.
- Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.
- Risk-free: product covered by our Quality 100% Guarantee.
Specifications
- T7 Promoter: 4605-4621
- LacO: 4627-4645
- T7 transcription start: 4621
- Cmyc: 4697-4735
- Prescission cleavage site: 4736-4760
- His6 Tag: 4768-4785
- T7 terminator: 4807-4855
- LacIq: 96-1175
- Ori pBR322: 2970-2356
- AMP R (Ampicillin resistance gene): 3133-3990
- f1 Origin: 4562-4115