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pColiExpress III Cloning & Expression Kits, 20 rxn-Plus

For a highly Efficient, Fast & Versatile Protein Expression (T4 DNA Ligase based) in E. coli from PCR fragment cloned

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Výrobca: CANVAX

Katalógové číslo: BE012

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Popis

pColiExpress™ III Cloning & Expression Kit

For a highly Efficient, Fast & Versatile Protein Expression (T4 DNA Ligase based) in E. coli from PCR fragment cloned

Product Overview:

pColiExpress™ III Cloning & Expression Kit is a DNA Cloning System for Protein Expression in E.coli. pColiExpress™ technology allows efficiently cloning DNA fragments and the quick production of a large quantity of any desired Protein.

pColiExpress™ III contains all elements required for periplasmic expression of any desired protein by OMPA Periplasmatic Signal peptide and T7 RNA Polymerase Inducible System. It gives a His6 tag at COOH end of the protein that allow the its purification. The vector has also an f1 origin of replication, an ampicillin resistance cassette, and a pBR322 origin of replication.

Advantages & Features

Ready-to-use vectors.
Highly efficient cloning system.
Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
Minimum background: lower than 1%.
Time-saving protocol: avoids any step required after PCR.
Higher protein expression than other suppliers.
Cost avoidance: avoids the use of expensive phosphorylated primers.
Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.
Risk-free: product covered by our Quality 100% Guarantee.

Specifications

T7 Promoter: 4605-4621
Lac O: 4627-4645
T7 transcription start: 4621
Signal Peptide OmpA: 4696-4777
His Tag: 4786-4803
T7 terminator: 4825-4872
LacIq: 96-1175
Ori pBR322: 2970-2356
Ampicillin resistance gene (ORF): 3133-3990
F1 Origin: 4562-4115