pColiExpress III LIC Cloning & Expression Kits, 20 rxn

pColiExpress III LIC Cloning & Expression Kits, 20 rxn

For a highly Efficient & Versatile Protein Expression (LIC technology based) in E. coli from PCR fragment cloned

Na objednávku
Výrobca: CANVAX
Katalógové číslo: BE010

pColiExpress™ III LIC Cloning & Expression Kit

For a highly Efficient & Versatile Protein Expression (LIC technology based) in E. coli from PCR fragment cloned

Product Overview:

pColiExpress™ III LIC Cloning & Expression Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family systems are based in directional ligase-independent-cloning (LIC) technology, a rapid procedure that provides high-cloning efficiencies and the fast production of a large quantity of any desired protein.

It contains all elements required for periplasmic expression of any desired protein by OMPA Periplasmatic Signal peptide and T7 RNA Polymerase Inducible System.  It includes a His6 tag at COOH end of the protein that allows the purification. The vector also has an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.

Advantages & Features

  • Ready-to-use vectors.
  • Highly efficient cloning system.
  • Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
  • Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
  • Minimum background: lower than 1%.
  • Time-saving protocol: avoids any step required after PCR.
  • Higher protein expression than other suppliers.
  • Cost avoidance: avoids the use of expensive phosphorylated primers.
  • Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

  • T7 Promoter: 4605-4621
  • LacO: 4627-4645
  • T7 transcription start: 4621
  • Signal Peptide OmpA: 4696-4777
  • His6 Tag: 4786-4803
  • T7 terminator: 4825-4872
  • LacIq: 96-1175
  • Ori pBR322: 2970-2356
  • AMP R (Ampicillin resistance gene): 3133-3990
  • f1 Origin: 4562-4115
Produkt bol pridaný do zoznamu prianí