pColiExpress I Cloning & Expression Kits, 20 rxn

pColiExpress I Cloning & Expression Kits, 20 rxn

For a highly Efficient, Fast & Versatile Protein Expression (T4 DNA Ligase based) in E. coli from PCR fragment cloned

Na objednávku
Výrobca: CANVAX
Katalógové číslo: BE002

pColiExpress™ I Cloning & Expression Kit

For a highly Efficient, Fast & Versatile Protein Expression (T4 DNA Ligase based) in E. coli from PCR fragment cloned

Product Overview:

pColiExpress™ I Cloning & Expression Kit is a DNA Cloning System for Protein Expression in E.coli.  pColiExpress™ technology allows efficiently cloning DNA fragments and the quick production of a large quantity of any desired Protein.

pColiExpress™ I contains all elements required for expression of large quantity of any desired protein by T7 RNA Polymerase Inducible System and a His6 tag at NH2 end that allow the protein purification. The vector has also an f1 origin of replication, an ampicillin resistance cassette, and a pBR322 origin of replication.

Advantages & Features

  • Ready-to-use vectors.
  • Highly efficient cloning system.
  • Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
  • Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
  • Minimum background: lower than 1%.
  • Time-saving protocol: avoids any step required after PCR.
  • Higher protein expression than other suppliers.
  • Cost avoidance: avoids the use of expensive phosphorylated primers.
  • Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

  • Features T7 Promoter: 4605-4621
  • Lac O: 4627-4645
  • T7 transcription start: 4621
  • His Tag: 4700-4717
  • T7 terminator: 4743-4791
  • LacIq: 96-1175
  • Ori pBR322: 2970-2356
  • Ampicillin resistance gene (ORF): 3133-3990
  • F1 Origin: 4562-411
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