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For a highly Efficient, Fast & Versatile Protein Expression (T4 DNA Ligase based) in E. coli from PCR fragment cloned
pColiExpress™ I Cloning & Expression Kit
For a highly Efficient, Fast & Versatile Protein Expression (T4 DNA Ligase based) in E. coli from PCR fragment cloned
Product Overview:
pColiExpress™ I Cloning & Expression Kit is a DNA Cloning System for Protein Expression in E.coli. pColiExpress™ technology allows efficiently cloning DNA fragments and the quick production of a large quantity of any desired Protein.
pColiExpress™ I contains all elements required for expression of large quantity of any desired protein by T7 RNA Polymerase Inducible System and a His6 tag at NH2 end that allow the protein purification. The vector has also an f1 origin of replication, an ampicillin resistance cassette, and a pBR322 origin of replication.
Advantages & Features
- Ready-to-use vectors.
- Highly efficient cloning system.
- Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
- Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
- Minimum background: lower than 1%.
- Time-saving protocol: avoids any step required after PCR.
- Higher protein expression than other suppliers.
- Cost avoidance: avoids the use of expensive phosphorylated primers.
- Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.
- Risk-free: product covered by our Quality 100% Guarantee.
Specifications
- Features T7 Promoter: 4605-4621
- Lac O: 4627-4645
- T7 transcription start: 4621
- His Tag: 4700-4717
- T7 terminator: 4743-4791
- LacIq: 96-1175
- Ori pBR322: 2970-2356
- Ampicillin resistance gene (ORF): 3133-3990
- F1 Origin: 4562-411