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PRIEMYSEL
- ! VÝPREDAJ !
Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest
pOnebyOne™ III – Lentiviral Bicistronic Mammalian Expression Kit
Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest
Product Overview:
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ III – Lentiviral incorporates the Human elongation factor 1 alpha promoter that precedes 2A sequence in frame with the truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones as they can be visualized by cytometry using a specific antibody labelled with FITC or using a similar method and also, they can be enriched from negative clones with magnetic beads bearing an anti-ΔNGFR antibody.
Advantages & Features
- Complete solution: a directional cloning vector to clone and produce a protein of interest.
- Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter).
- Highly efficient: cloning system tested with up to 4 kb inserts.
- Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment.
- Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest.
- Cost avoidance: avoids the use of restriction enzymes.
- Accurate: proven performance for most common cell lines.
- Flexible: allows transfection in difficult cell lines.
- Really low experimental background: lower than 1%.
- Convenient: available with different resistance marker cassettes.
- Directional cloning: of PCR with the gene of interest.
- Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.
- Risk-free: product covered by our Quality 100% Guarantee.
Includes
Includes for 20 rxn:
– 20 µL Linearized vector (50 ng/µL)
– 40 µL Glue Enzyme (10 u/µL)
– 50 µL Glue Enzyme Buffer (10x)
– 10 µL Insert Control (30 ng/µL)
– 5 µL pOnebyOne™ Control (50 ng/µL)