The anti-APP antibody [pT668] recognizes the phosphorylated threonine 668 of Amyloid Protein Precursor (APP). This antibody is suitable for Western Blot, for Immunohistochemistry and Immunofluorescence.

This polyserum was obtained by immunizing a rabbit with a 12 amino acid peptide containing the phosphorylated threonine 668 (YVDAAVTPEERH). This antibody is determined by its ability to recognize human APP phosphorylated at threonine 668.

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Background on the anti-Tau antibody phosphorylated at threonine 668

Amyloid Protein Precursor also called APP (Gene ID: 351) is a type 1 transmembrane protein whose metabolism leads to the production of amyloid-beta peptides. In Alzheimer’s disease, amyloid peptides are the main components of amyloid deposits. Amyloid-beta peptides (Ab) are produced following by sequential cleavages of APP by proteases that are named secretases.

The phosphorylation of Anti-APP antibody at threonine 668 (a position corresponding to the APP695 isoform) is increased in Alzheimer’s disease and promotes amyloid-beta peptide production.

APP is phosphorylated at Thr-668 both in vitro and in vivo by a number of kinases including glycogen synthase kinase 3β (GSK3β), Jun N-terminal kinase-3 (JNK3), Jun N-terminal kinase 1 (JNK1), cell division cycle protein (Cdc2), cyclin-dependent kinase 5 (Cdk5) and DYRK1A (Dual-specificity tyrosine(Y)-phosphorylation regulated kinase 1A).

Phosphorylation of APP at Thr-668 results in significant conformational changes and is associated with neurite extension, anterograde transport of vesicular cargo into neurites, axonal transport and in signaling to the nucleus.

4BioDx polyclonal antibodies against APP at phosphorylated Thr-668 (4BDX-1503S) are useful tools to detect phosphorylated APP in rat, mice or human brain homogenates by western blot. These antibodies worked greatly in immunohistochemistry and immunofuorescence protocols and they indicated neuritic processes surrounding amyloid deposits (see applications below).

The Anti-APP [pT668] antibody (Ref. 4BDX-1503S) recognizes the phosphorylated threonine 668 of Amyloid Protein Precursor (APP). This antibody is a biomarker of neuritic processes surrounding amyloid deposits.