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CVX5α™ Chemically Competent Cells
Versatile, Convenient & Cost-effective solution for routine Subcloning Procedures
Product Overview:
CVX5α™ Chemically Competent Cells is a versatile, convenient and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane.
CVX5α™ Genotype: F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ).
Advantages & Features
- Versatile: proven performance for high-efficiency transformation in a wide variety of applications.
- Convenient: ideal for routine.
- Compatible: with blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal.
- Cost avoidance: dry ice free of charge.
Quality Control
- Each lot of competent cells is tested to verify transformation efficiencies using 100 pg pUC18 supercoiled DNA and the recommended protocol.
- Under these conditions, transformation efficiency will be ≥ 1 x 107 cfu/μg pUC18.
- Transformation efficiency is defined as the number of colony forming units (cfu) produced by transforming 1 μg of plasmid (3 kb) into a given volume of competent cells.