Klonovací systém pSpark® III

For highly Efficient, Accurate & Easy Cloning with Ampicillin and Kanamycin resistance

Product Overview:

pSpark® III is a highly efficient, accurate and easy-to-use DNA cloning system that combines Ampicillin and Kanamycin resistance. Ideal for cloning PCR products amplified from any plasmid vector without the need to gel-purify bands to eliminate the background due to the template vector used for PCR.

• Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
• Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
• Powerful: obtain 5-fold more positive colonies using 10x less DNA insert.
• Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding “suicide” strategies from toxic genes.
• High stability: eliminates cloning bias or pitfalls.
• Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
• Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
• High cost-saving:  reduces your cloning costs as no expensive phosphorylated primers are needed.
• Eliminates positive selection vector.
• Risk-free: product covered by our Quality 100% Guarantee.