Klonovací systém pSpark® I

Klonovací systém pSpark® I

DNA cloning

Na objednávku
Výrobca: CANVAX
Katalógové číslo: C0001-S

Obr. Názov variantu Cena Stav Objednať
Počet reakcií : 5 rxn
0,00 €
Na objednávku
Počet reakcií : 10 rxn
101,64 €
Na objednávku
Počet reakcií : 20 rxn
197,51 €
Na objednávku

pSpark® I DNA Cloning Kit

For highly Efficient, Accurate & Robust general Cloning from PCR High Fidelity fragments, without toxic genes use

Product Overview:

pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency.

The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

  • Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
  • Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding “suicide” strategies from toxic genes.
  • Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
  • High stability: eliminates cloning bias or pitfalls.
  • Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert.
  • Compatible with blue/white screening.
  • Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
  • Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
  • Eliminates positive selection vector.
  • High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed.
  • Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
  • Risk-free: product covered by our Quality 100% Guarantee.
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